Step 2: Enter input.

Enter gi number (e.g. 157835681):

Show Advanced Options

Advanced Input and Parameters (Optional)
Trace Options
Real-Valued Trace (rvET)
Position-specific gap-reducing real-valued trace
BLAST
E-value cutoff:
Number of proteins to extract:
Use PSI-BLAST
Number of PSI-BLAST iterations:
Use all sequences from all iterations of PSI-BLAST
BLAST Database Options
Custom NCBI
Custom UniRef90
Custom UniRef100
Custom SwissProt reviewed
Sequence Selection
Restrict BLAST hits to gi#'s:
Restrict BLAST hits to # proteins:
Restrict BLAST hits to minimum e-value:
Restrict BLAST hits to maximum e-value:
Restrict BLAST hits to minimum sequence identity:
Restrict BLAST hits to maximum sequence identity:
Restrict BLAST hits to minimum fractional length to query:
Alignment & Tree
Do not remove fragments
Fractional length used to define fragments (in relation to the query in the alignment):
Do not remove identical sequences
Do not remove short sequences
Fractional length used to define short sequences (in relation to the query):
Tree File (NHX format):
Custom Sequence Input (if not using BLAST)
Alignment File (GCG format):
-Or- Sequences File (FASTA format):
Query name in input file (eg: query_1aca):

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