Universal Evolutionary Trace

Step 2: Enter input.

(Load example input)

Enter short name or ID (Reference sequence will be named query_ID):

Enter or paste the amino acid sequence in FASTA format below. Only the one-letter codes of the 20 standard amino acids, upper or lower case (ARNDCEQGHILKMFPSTWYVarndceqghilkmfpstwyv), will be accepted:
MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGP DEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAK SVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHE RCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNS SCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELP PGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPG GSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD

Show Advanced Options

Blast Options Alignment or Sequence File

Advanced Input and Parameters (Optional)
Trace Options
Real-Valued Trace (rvET)
Optimize selection of sequences from alignment for rvET
Position-specific gap-reducing real-valued trace
pair-interaction ET (piET)
BLAST
E-value cutoff:
Number of proteins to extract:
Use PSI-BLAST
Number of PSI-BLAST iterations:
Use all sequences from all iterations of PSI-BLAST
BLAST Database Options
Custom NCBI
Custom UniRef90
Custom UniRef100
Custom SwissProt reviewed
Sequence Selection
Restrict BLAST hits to gi#'s:
Restrict BLAST hits to # proteins:
Restrict BLAST hits to minimum e-value:
Restrict BLAST hits to maximum e-value:
Restrict BLAST hits to minimum sequence identity:
Restrict BLAST hits to maximum sequence identity:
Restrict BLAST hits to minimum fractional length to query:
Do not remove fragments
Fractional length used to define fragments (in relation to the query in the alignment):
Do not remove identical sequences
Do not remove short sequences
Fractional length used to define short sequences (in relation to the query):

Advanced Input and Parameters (Optional)
Trace Options
Real-Valued Trace (rvET)
Optimize selection of sequences from alignment for rvET
Position-specific gap-reducing real-valued trace
pair-interaction ET (piET)
Alignment File (GCG/MSF format):
-Or- Sequences File (FASTA format):
Query name in input file (eg: query_1aca):
Tree File (NHX format)(Optional):

Note: The output of the new trace will not include structure data.